In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

Diabetes imaging—quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Coherence Microscopy (xfOCM). To identify and quantify the islets of Langerhans observed in xfOCM tomograms we implemented an active contour algori...

Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy.

We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended-focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amyloid plaques in the brain parenchyma and vasculature and requires no staining of the alzheimeric sample under investigation. xfOCM also opens t...

In Vivo Imaging of Pathological Features of Alzheimer’s Disease in a Mouse Model Using Extended-focus Optical Coherence Microscopy

Extended focus depth for Fourier domain optical coherence microscopy.

We report on a new detection scheme for Fourier domain optical coherence microscopy that exhibits high transverse resolution along an axially extended focal range. Nearly constant transverse resolution of approximately 1.5 microm along a focal range of 200 microm is experimentally verified with a maximum sensitivity of 105 dB. A broad-bandwidth Ti:sapphire laser allowed for an axial resolution ...

Integrated optical coherence tomography and optical coherence microscopy imaging of ex vivo human renal tissues.

PURPOSE We evaluated the feasibility of using optical coherence tomography and optical coherence microscopy technology to assess human kidney morphology. MATERIALS AND METHODS A total of 35 renal specimens from 19 patients, consisting of 12 normal tissues and 23 tumors (16 clear cell renal cell carcinomas, 5 papillary renal cell carcinomas and 2 oncocytomas) were imaged ex vivo after surgical...